Phospho-FOXO1 (Ser319) Rekombinanter Antikörper

Phospho-FOXO1 (Ser319) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250275C3

Kat-Nr. : 82997-7-RR

Synonyme

FOXO1 (Ser319), p FOXO1 (Ser319), p-FOXO1, p-FOXO1 (Ser319), p-FOXO1(Ser319)

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  WB analysis using 82997-7-RR

  HEK-293T and MCF-7 cell lysates were subjected to SDS PAGE followed by western blot with 82997-7-RR (Phospho-FOXO1 (Ser319) antibody) blocked with BSA only, Phospho-FOXO1 (Ser319) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82997-7-RR bindet in WB, ELISA Phospho-FOXO1 (Ser319) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: forkhead box O1
• Berechnetes Molekulargewicht: 655 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC021981
• Gene symbol: FOXO1
• Gene ID (NCBI): 2308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593)