Phospho-FRS2 (Tyr436) Polyklonaler Antikörper

Phospho-FRS2 (Tyr436) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31352-1-AP

Synonyme

p FRS2 , p FRS2 (Tyr436), p FRS2 Tyr436, p-FRS2 , p-FRS2 (Tyr436)

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  WB analysis using 31352-1-AP

  Non-treated NIH/3T3 cells and bFGF (HZ-1285) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 31352-1-AP (Phospho-FRS2 (Tyr436) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bFGF treated NIH/3T3 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31352-1-AP bindet in WB, ELISA Phospho-FRS2 (Tyr436) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fibroblast growth factor receptor substrate 2
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 80-85 kDa
• GenBank-Zugangsnummer: BC021562
• Gene symbol: FRS2
• Gene ID (NCBI): 10818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor substrate 2 (FRS2), a lipid-anchored docking protein that is phosphorylated upon activation of FGFR, is critical for recruitment of downstream signaling molecules and links the FGFRs to the Ras/Mek/Erk pathway and the PI3-Kinase/Akt pathway. Studies have found that the phosphotyrosine binding domain of FRS-2 directly binds the Trk receptors at the same phosphotyrosine residue that binds the signaling adapter Shc, suggesting a model in which competitive binding between FRS-2 and Shc regulates differentiation versusproliferation. (PMID: 19053057, PMID: 10092678)