Phospho-FRS2 (Tyr436) Rekombinanter Antikörper

Phospho-FRS2 (Tyr436) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242134H8

Kat-Nr. : 85242-1-PBS

Synonyme

P FRS2 (Tyr436), P-FRS2 (Tyr436), Phos FRS2 (Tyr436), Phos-FRS2 (Tyr436), Phospho-FRS2-α (Tyr436)

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  WB analysis of NIH/3T3 using 85242-1-RR (same clone as 85242-1-PBS)

  Non-treated NIH/3T3 cells and bFGF (HZ-1285) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85242-1-RR (Phospho-FRS2 (Tyr436) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 85242-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85242-1-PBS bindet in WB, Indirect ELISA Phospho-FRS2 (Tyr436) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fibroblast growth factor receptor substrate 2
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 80-85 kDa
• GenBank-Zugangsnummer: BC021562
• Gene symbol: FRS2
• Gene ID (NCBI): 10818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor substrate 2 (FRS2), a lipid-anchored docking protein that is phosphorylated upon activation of FGFR, is critical for recruitment of downstream signaling molecules and links the FGFRs to the Ras/Mek/Erk pathway and the PI3-Kinase/Akt pathway. Studies have found that the phosphotyrosine binding domain of FRS-2 directly binds the Trk receptors at the same phosphotyrosine residue that binds the signaling adapter Shc, suggesting a model in which competitive binding between FRS-2 and Shc regulates differentiation versusproliferation. (PMID: 19053057, PMID: 10092678)