Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Rekombinanter Antikörper

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242663E11

Kat-Nr. : 82371-5-PBS

Synonyme

P-GSK3A (Tyr279), P-GSK3B (Tyr216), Phos-GSK3 beta (Tyr216), Phos-GSK3A (Tyr279), Phos-GSK3B (Tyr216)

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  WB analysis using 82371-5-RR (same clone as 82371-5-PBS)

  HEK-293 cells and NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82371-5-RR (Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody) blocked with BSA only, Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) peptide or non-Phospho peptide at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 82371-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82371-5-PBS bindet in WB, Indirect ELISA Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)