Phospho-GSK3B (Ser9) Monoklonaler Antikörper

Phospho-GSK3B (Ser9) Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9E2

Kat-Nr. : CL488-67558

Synonyme

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta, Phospho-GSK3B (Ser9)

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Galerie der Validierungsdaten

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  IF Staining of PC-3 using CL488-67558

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated PC-3 cells and non-treated PC-3 cells using CoraLite® Plus 488 Phospho-GSK3B (Ser9) antibody (CL488-67558, Clone: 1C9E2 ) at dilution of 1:100.

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  FC experiment of PC-3 using CL488-67558

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-GSK3B (Ser9) (CL488-67558, Clone:1C9E2), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Mit Calyculin A behandelte PC-3-Zellen
• Erfolgreiche Detektion in FC: Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67558 bindet in IF, FC (Intra) Phospho-GSK3B (Ser9) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glycogen synthase kinase 3 beta
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: NM_002093
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .