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Phospho-GSK3B (Ser9) Monoklonaler Antikörper

Phospho-GSK3B (Ser9) Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C9E2

Kat-Nr. : CL647-67558

Synonyme

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta, Phospho-GSK3B (Ser9)

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 647-conjugated Phospho-GSK3B (Ser9) (CL647-67558)

    FC experiment of PC-3 using CL647-67558

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.125 ug CoraLite® Plus 647 Anti-Human Phospho-GSK3B (Ser9) (CL647-67558, Clone:1C9E2) (red), or 0.125 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-67558 bindet in FC (Intra) Phospho-GSK3B (Ser9) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name glycogen synthase kinase 3 beta
Beobachtetes Molekulargewicht 48 kDa
GenBank-ZugangsnummerNM_002093
Gene symbol GSK3B
Gene ID (NCBI) 2932
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .