Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Polyklonaler Antikörper

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (2)

Kat-Nr. : 29125-1-AP

Synonyme

GSK3B (Tyr216), p GSK3B , p GSK3B (Tyr216), p GSK3B Tyr216, p-GSK3A (Tyr279)

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  WB analysis using 29125-1-AP

  Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29125-1-AP (Phospho-GSK3B (Tyr216) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29125-1-AP

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29125-1-AP (Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• ELISA: See 1 publications below

Produktinformation

29125-1-AP bindet in WB, ELISA Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)

Publikationen

Species	Application	Title
mouse	WB	Exp Neurol; Inhibition of histamine receptor 3 alleviates sevoflurane-induced hypomyelination and neurobehavioral deficits; Authors - Ji Che
mouse	WB	Neurochem Res; Involvement of PI3K/AKT Pathway in the Rapid Antidepressant Effects of Crocetin in Mice with Depression-Like Phenotypes; Authors - Susu Lin
rat	WB	Evid Based Complement Alternat Med; Sufentanil Alleviates Sepsis-Induced Myocardial Injury and Stress Response in Rats through the ERK/GSK-3β Signaling Axis.; Authors - Hongcheng Zang
human	WB	Mar Drugs; Antidiabetic Effect of Collagen Peptides from Harpadon nehereus Bones in Streptozotocin-Induced Diabetes Mice by Regulating Oxidative Stress and Glucose Metabolism; Authors - Qianxia Lin
mouse	WB	Front Aging Neurosci; The γ-Adducin 1-357 fragment promotes tau pathology; Authors - Honglu Yu
mouse	WB	Aging (Albany NY); The Wnt/β-catenin signaling pathway inhibits osteoporosis by regulating the expression of TERT: an in vivo and in vitro study; Authors - Yuanqing Cai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (06-25-2025)

Works nicely with my WB.

• Applications: Western Blot

FH

Patricia (Verified Customer) (01-31-2025)

Great Ab. Gave clean results.

• Applications: Western Blot