Phospho-GYS (Ser641) Polyklonaler Antikörper

Phospho-GYS (Ser641) Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 28855-1-AP

Synonyme

GYS2, p GYS1 , p GYS1 (Ser641), p GYS1 Ser641, p-GYS1

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  WB analysis using 28855-1-AP

  Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase PC-3 cells were subjected to SDS PAGE followed by western blot with 28855-1-AP (Phospho-GYS1 (Ser641) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of PC-3 using 28855-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated PC-3 cells using Phospho-GYS1 (Ser641) antibody (28855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: λ phosphatase treated PC-3 cells
• Erfolgreiche Detektion in IF/ICC: λ phosphatase treated PC-3 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

28855-1-AP bindet in WB, IF/ICC, ELISA Phospho-GYS (Ser641) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glycogen synthase 1 (muscle)
• Berechnetes Molekulargewicht: 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC007688
• Gene symbol: GYS1
• Gene ID (NCBI): 2997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in the liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites, and insulin stimulates the dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity, which will decrease phosphorylation of GS and increase glycogen synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID:22232606)

Publikationen

Species	Application	Title
mouse	WB	J Ethnopharmacol; Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis; Authors - Xu Yang