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Phospho-GYS (Ser641) Rekombinanter Antikörper

Phospho-GYS (Ser641) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250744A11

Kat-Nr. : 80102-2-PBS

Synonyme

GYS2, P GYS1 (Ser641), P-GYS1 (Ser641), Phos GYS1 (Ser641), Phos-GYS1 (Ser641)

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-GYS (Ser641) Recombinant antibody (80102-2-RR)

    WB analysis using 80102-2-RR (same clone as 80102-2-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated and λ phosphatase NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80102-2-RR (Phospho-GYS (Ser641) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 80102-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

80102-2-PBS bindet in WB, Indirect ELISA Phospho-GYS (Ser641) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name glycogen synthase 1 (muscle)
Berechnetes Molekulargewicht 84 kDa
Beobachtetes Molekulargewicht 84 kDa
GenBank-ZugangsnummerBC007688
Gene symbol GYS1
Gene ID (NCBI) 2997
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in the liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites, and insulin stimulates the dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity, which will decrease phosphorylation of GS and increase glycogen synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID:22232606)