Phospho-HER2/ErbB2 (Tyr877) Rekombinanter Antikörper

Phospho-HER2/ErbB2 (Tyr877) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250539F5

Kat-Nr. : 82762-11-PBS

Synonyme

ErbB2 (Tyr877), HER2 (Tyr877), HER2/ErbB2 (Tyr877), P-HER2/ErbB2, P-HER2/ErbB2 (Tyr877)

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  WB analysis using 82762-11-RR (same clone as 82762-11-PBS)

  Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 82762-11-RR (Phospho-HER2/ErbB2 (Tyr877) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82762-11-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82762-11-PBS bindet in WB, Indirect ELISA Phospho-HER2/ErbB2 (Tyr877) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Berechnetes Molekulargewicht: 1255 aa, 138 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC156755
• Gene symbol: HER2/ErbB2
• Gene ID (NCBI): 2064
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr877) refers to the phosphorylated form of the human epidermal growth-factor receptor 2 (HER2, ErbB2) at tyrosine 877 within its kinase domain. This phosphorylation event is catalyzed in trans by dimerization-activated HER2 itself or by Src-family kinases (SFKs) upon receptor clustering, and serves as a critical activation switch for downstream signaling cascades. ( PMID: 34107301)