Phospho-Hamartin/TSC1 (Ser511) Rekombinanter Antikörper

Phospho-Hamartin/TSC1 (Ser511) Rekombinant Antikörper für Indirect ELISA, WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

Indirect ELISA, WB

Konjugation

Unkonjugiert

CloneNo.

3N6

Kat-Nr. : 80340-1-PBS

Synonyme

TSC1, TSC 1, TSC, LAM, KIAA0243

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  WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

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  WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

  Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 80340-1-RR (same clone as 80340-1-PBS)

  1X10^6 HeLa cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80340-1-PBS bindet in Indirect ELISA, WB Phospho-Hamartin/TSC1 (Ser511) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tuberous sclerosis 1
• Berechnetes Molekulargewicht: 130 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_000368
• Gene symbol: Hamartin/TSC1
• Gene ID (NCBI): 7248
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).