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Phospho-Histone H2A (Thr120) Rekombinanter Antikörper

Phospho-Histone H2A (Thr120) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250365B1

Kat-Nr. : 83041-8-PBS

Synonyme

Histone H2A (Thr120), p Histone H2A, p Histone H2A (Thr120), p Histone H2A Thr120, p-Histone H2A

Filter:
  • Western Blot (WB) analysis of HeLa cells using Phospho-Histone H2A (Thr120) Recombinant monoclona (83041-8-RR)

    WB analysis of HeLa using 83041-8-RR (same clone as 83041-8-PBS)

    Nocodazole and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 83041-8-RR (Phospho-Histone H2A (Thr120) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 83041-8-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

83041-8-PBS bindet in WB, Indirect ELISA Phospho-Histone H2A (Thr120) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name histone cluster 1, H2ae
Berechnetes Molekulargewicht 14 kDa
Beobachtetes Molekulargewicht15 kDa
GenBank-ZugangsnummerBC093836
Gene symbol HIST1H2AE
Gene ID (NCBI) 3012
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospho-Histone H2A (Thr120) refers to the phosphorylation of threonine 120 on histone H2A, a modification that is increasingly recognized for its roles in chromatin organization, gene regulation, and disease development, particularly in oncogenesis and bone remodeling. The phosphorylation is predominantly associated with gene silencing. High levels of H2AT120p correlate with increased cell proliferation, tumor growth, and poor prognosis, underscoring its role as an oncogenic signal.