Phospho-Histone H2A.X (Ser139) Polyklonaler Antikörper

Phospho-Histone H2A.X (Ser139) Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 29380-1-AP

Synonyme

H2A.X (Ser139), H2A.X Ser139, Histone H2A.X (Ser139), Histone H2A.X Ser139, p Histone H2A.X

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  WB analysis using 29380-1-AP

  Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 29380-1-AP (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Histone H2A.X antibody and Histone H3 antibody as loading control.

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  IF Staining of U2OS using 29380-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Phospho-Histone H2A.X (Ser139) antibody (29380-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: UV treated HEK-293 cells
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 4 publications below

Produktinformation

29380-1-AP bindet in WB, IF/ICC, ELISA Phospho-Histone H2A.X (Ser139) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: H2A histone family, member X
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC013416
• Gene symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)

Publikationen

Species	Application	Title
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB	J Nanobiotechnology; Synergistic cerium oxide nanozymes: targeting DNA damage and alleviating tumor hypoxia for improved NSCLC radiotherapy efficiency; Authors - Jie Liu
human	WB	Bioorg Chem; Dual inhibition of oxidative phosphorylation and glycolysis to enhance cancer therapy; Authors - Xi Sheng
mouse	IF	Ecotoxicol Environ Saf; 2-bromoacetamide exposure impairs oocyte maturation in mice and humans primarily via disrupting the cytoskeleton; Authors - Chuanxin Zhang
human	WB	Eur J Med Chem; Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation; Authors - Amandeep Thakur
human	WB	Mol Cell; A TBK1-independent primordial function of STING in lysosomal biogenesis; Authors - Bo Lv