Phospho-Histone H2A.X (Ser139) Rekombinanter Antikörper

Phospho-Histone H2A.X (Ser139) Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

5N19

Kat-Nr. : 83307-2-RR

Synonyme

H2A.X (Ser139), H2A.X P-S139, H2A.X Ser139, H2A.XS139, H2A.XS139Ph

---

• 

  WB analysis using 83307-2-RR

  Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control.

• 

  WB analysis of C6 using 83307-2-RR

  C6 cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of Jurkat using 83307-2-RR

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Jurkat using 83307-2-RR

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 83307-2-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 83307-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 83307-2-RR

  Immunofluorescent analysis of (4% PFA) fixed UV treated HeLa cells using Phospho-Histone H2A.X (Ser139) antibody (83307-2-RR, Clone: 5N19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of Jurkat using 83307-2-RR

  1x10^6 Jurkat cells untreated (dashed lines) or treated with Staurosporine which intracellularly stained with 0.06 ug Phospho-Histone H2A.X (Ser139) Recombinant antibody (83307-2-RR, Clone:5N19) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Staurosporin behandelte Jurkat-Zellen, C6-Zellen
• Erfolgreiche Detektion in IHC: Jurkat-Zellen, Mausmilzgewebe, Rattenmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: UV treated HeLa cells
• Erfolgreiche Detektion in FC (Intra): mit Staurosporin behandelte Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

83307-2-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Phospho-Histone H2A.X (Ser139) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: H2A histone family, member X
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC013416
• Gene symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)

Publikationen

Species	Application	Title
human	WB	Transl Oncol; PiR-hsa-23533 promotes malignancy in head and neck squamous cell carcinoma via USP7; Authors - Hanlin Hu
human	IF	J Microbiol Biotechnol; BlingLife®-Marigold Extract Alleviates Blue Light-Induced Retinal Mitochondria Oxidative Stress and Senescence by Activating NRF2/HO-1 Signaling; Authors - Zixiu Zhou