Phospho-Histone H3 (Ser10) Rekombinanter Antikörper

Phospho-Histone H3 (Ser10) Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

242921B1

Kat-Nr. : 82828-10-RR

Synonyme

H3 P-S10, H3S10, H3S10Ph, Histone H3 (Ser10), p Histone H3

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  WB analysis of HeLa using 82828-10-RR

  Nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.

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  WB analysis of NIH/3T3 using 82828-10-RR

  Nocodazole treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  WB analysis of HSC-T6 using 82828-10-RR

  Calyculin A treated and untreated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  IHC staining of mouse brain using 82828-10-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 82828-10-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4, red).

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  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4, red).

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  ChIP experiment of HeLa using 82828-10-RR

  Chromatin was prepared from HeLa cells either non-treated or treated with Nocodazole (100 ng/ml, 16 h). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Ser10) (82828-10-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: nocodazole treated HeLa cells, mit Calyculin A behandelte HSC-T6-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in ChIP-qPCR: Nocodazole (100 ng/ml, 16 h) HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82828-10-RR bindet in WB, IHC, IF/ICC, ELISA, ChIP-qPCR Phospho-Histone H3 (Ser10) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: NM_003529
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.