Phospho-Histone H3 (Ser28) Rekombinanter Antikörper

Phospho-Histone H3 (Ser28) Rekombinant Antikörper für WB, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

250369A1

Kat-Nr. : 82828-15-RR

Synonyme

Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28, p-Histone H3

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  WB analysis of HeLa using 82828-15-RR

  Nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 82828-15-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

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  ChIP experiment of HeLa using 82828-15-RR

  Chromatin was prepared from HeLa cells treated with Nocodazole (100 ng/ml) for 16 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Ser28) (82828-15-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: nocodazole treated HeLa cells
• Erfolgreiche Detektion in ChIP-qPCR: Nocodazole-HeLa (100 ng/ml, 16 h) HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82828-15-RR bindet in WB, ELISA, ChIP-qPCR Phospho-Histone H3 (Ser28) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone modifications and variants have key roles in the activation and silencing of genes. Phosphorylation of histone H3 at serine 10 and serine 28 is involved in transcriptional activation of genes responding to stress or mitogen-stimulated signaling pathways. Histone H3 phosphorylated at serine 28 was located at the promoter region of the transcriptionally active, but not competent, histone H5 and β-globin genes (PMID: 17913747).