Phospho-Histone H3 (Thr3) Rekombinanter Antikörper

Phospho-Histone H3 (Thr3) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

241897G3

Kat-Nr. : 84739-1-PBS

Synonyme

H3 P-T3, H3T3, H3T3Ph, Histone H3 (Thr3), p Histone H3

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  WB analysis using 84739-1-RR (same clone as 84739-1-PBS)

  Non-treated HeLa cels, NIH/3T3 cells, HSC-T6 cells, and Calyculin A treated HeLa cels, NIH/3T3 cells, HSC-T6 cells were subjected to SDS PAGE followed by western blot with 84739-1-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84739-1-RR, Clone: 241897G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR, Clone:241897G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Thr3) (84739-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84739-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR Phospho-Histone H3 (Thr3) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.