Phospho-IRAK4 (Thr345/Ser346) Rekombinanter Antikörper

Phospho-IRAK4 (Thr345/Ser346) Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242271A8

Kat-Nr. : 85753-1-RR

Synonyme

IRAK4 (Ser346), IRAK4 (Thr345), IRAK4 (Thr345/Ser346), P IRAK4 (Thr345/Ser346), Phospho IRAK4 (Thr345/Ser346)

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  WB analysis of Karpas-299 using 85753-1-RR

  Non-treated Karpas-299 cells and IL-1 beta treated Karpas-299 cells were subjected to SDS PAGE followed by western blot with 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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  IHC staining of rat lymph node using 85753-1-RR

  Immunohistochemical analysis of paraffin-embedded rat lymph node tissue slide using 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lymph node using 85753-1-RR

  Immunohistochemical analysis of paraffin-embedded rat lymph node tissue slide using 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: IL-1 beta treated Karpas-299 cells
• Erfolgreiche Detektion in IHC: rat lymph node tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85753-1-RR bindet in WB, IHC, ELISA Phospho-IRAK4 (Thr345/Ser346) und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: interleukin-1 receptor-associated kinase 4
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC013316
• Gene symbol: IRAK4
• Gene ID (NCBI): 51135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRAK4 is the central kinase in the Toll-like receptor pathway of the innate immune system. Toll-like receptors constitute the first line of defense against pathogenic micro-organisms such as bacteria, viruses, and yeast. Unphosphorylated full-length IRAK4 can autophosphorylate in vitro in a cell-free system. In the cellular context, however, autophosphorylation is inducible by IL-1β. Four phosphorylation sites have been identified in the activation loop in some articles, which are the results of autophosphorylation: Thr-342, Thr-345, Ser-346 and Thr-352. (PMID: 24567333)