Phospho-IRF3 (Ser396) Polyklonaler Antikörper

Phospho-IRF3 (Ser396) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 29528-1-AP

Synonyme

interferon regulatory factor 3, IRF 3, IRF3, Phospho-IRF3 (Ser396)

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Galerie der Validierungsdaten

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  WB analysis using 29528-1-AP

  Untreated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29528-1-AP (Phospho-IRF3 (Ser396) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS behandelte THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

29528-1-AP bindet in WB, ELISA Phospho-IRF3 (Ser396) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: interferon regulatory factor 3
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC009395
• Gene symbol: IRF3
• Gene ID (NCBI): 3661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferons (IFNs) are a large family of multifunctional secreted proteins involved in antiviral defense, cell growth regulation, and immune activation(PMID: 18440553). A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation. After cytoplasmic IRF-3 is phosphorylated at Ser386 and Ser396, phosphorylated IRF-3 translocates into the nucleus. This phosphorylated IRF-3 activates IFN and IFN-responsive genes.

Publikationen

Species	Application	Title
pig	WB	Int J Biol Macromol; Role of OGDH in Atophagy-IRF3-IFN-β pathway during classical swine fever virus infection; Authors - Sen Zeng
human	WB	Int J Mol Sci; FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3; Authors - Zhiqiang Xu
	WB	Signal Transduct Target Ther; The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation; Authors - Weijie Ouyang
monkey	WB	Viruses; Naringenin Improves Innate Immune Suppression after PRRSV Infection by Reactivating the RIG-I-MAVS Signaling Pathway, Promoting the Production of IFN-I; Authors - Jiaying Yu
mouse	WB	Inflammopharmacology; Rapamycin can alleviate the submandibular gland pathology of Sjögren's syndrome by limiting the activation of cGAS-STING signaling pathway; Authors - Wen Zhu