Phospho-IRS1 (Ser307) Rekombinanter Antikörper

Phospho-IRS1 (Ser307) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242124E4

Kat-Nr. : 85238-1-PBS

Synonyme

IRS1 (Ser307), P IRS1 (Ser307), Phospho IRS1, Phospho IRS1 (Ser307), Phospho-IRS1

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  WB analysis using 85238-1-RR (same clone as 85238-1-PBS)

  Non-treated NIH/3T3 cells and TNF alpha (HZ-1014) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85238-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85238-1-RR (same clone as 85238-1-PBS)

  Non-treated and TNF alpha (HZ-1014) treated HeLa cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85238-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85238-1-PBS bindet in WB, Indirect ELISA Phospho-IRS1 (Ser307) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: insulin receptor substrate 1
• Berechnetes Molekulargewicht: 1242 aa, 132 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC053895
• Gene symbol: IRS1
• Gene ID (NCBI): 3667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307/312, Ser267/270, and Ser612/616 in rodent/human IRS-1. (PMID: 11606564, PMID: 12714600)