Phospho-IkB Alpha (Ser32/36) Rekombinanter Antikörper

Phospho-IkB Alpha (Ser32/36) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1G21

Kat-Nr. : 82349-1-RR

Synonyme

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA, Phospho-IkB Alpha (Ser32/36)

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 82349-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha-tubulin antibody as loading control.

• 

  WB analysis using 82349-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82349-1-RR

  PMA treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HeLa-Zellen, PMA treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

82349-1-RR bindet in WB, ELISA Phospho-IkB Alpha (Ser32/36) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004983
• Gene symbol: NFKBIA
• Gene ID (NCBI): 4792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha). (PMID: 16904979, PMID: 28990531 PMID: 10455908)

Publikationen

Species	Application	Title
human	WB	Transl Oncol; Endogenous hydrogen sulfide inhibition suppresses tumor growth by promoting apoptosis and pyroptosis in esophageal cancer cells; Authors - Hong-Gang Wang
rat	WB	Phytomedicine; Ganoderma lucidum polysaccharide peptide (GLPP) attenuates rheumatic arthritis in rats through inactivating NF-κB and MAPK signaling pathways; Authors - Meng Meng
human	WB	Mol Immunol; Catapol attenuates the aseptic inflammatory response to hepatic I/R injury in vivo and in vitro by inhibiting the HMGB1/TLR-4/NF-κB signaling pathway via the microRNA-410-3p; Authors - Zan Jie Feng
human	WB	Am J Physiol Cell Physiol; Inducible nitric oxide synthase activity mediates TNF-α-induced endothelial cell dysfunction; Authors - Chen Liu
human,rat	WB	J Ethnopharmacol; The Terminalia chebula Retz extract treats hyperuricemic nephropathy by inhibiting TLR4/MyD88/NF-κB axis; Authors - Hao Liu
human,mouse	WB	Int J Biol Macromol; In vitro and in vivo immunomodulatory activity of acetylated polysaccharides from Cyclocarya paliurus leaves; Authors - Yang Zhang