Phospho-JAK3 (Tyr980/981) Polyklonaler Antikörper

Phospho-JAK3 (Tyr980/981) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 29101-1-AP

Synonyme

JAK 3, JAK3, JAK3_HUMAN, JAKL, Janus kinase 3, L JAK, Leukocyte janus kinase, LJAK, Phospho-JAK3 (Tyr980/981), Tyrosine protein kinase JAK3

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  WB analysis using 29101-1-AP

  Non-treated and IL-4 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 29101-1-AP (Phospho-JAK3 (Tyr980/981) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: IL-4 treated HepG2 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

29101-1-AP bindet in WB, ELISA Phospho-JAK3 (Tyr980/981) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• Berechnetes Molekulargewicht: 1124 aa, 125 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC028068
• Gene symbol: JAK3
• Gene ID (NCBI): 3718
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. Janus kinase family comprise four tyrosine (Tyr) kinases (JAK1, JAK2, JAK3 and Tyk2). In the cytoplasm, JAKs play a pivotal role in signal transduction via its association with type I and type II cytokine receptors, which include receptors for interleukins (ILs), interferons (IFNs), and hormones such as leptin. Interaction of a cytokine with its membrane-bound receptor complex triggers activation of receptor-bound JAKs, which phosphorylate the receptor on key cytoplasmic Tyr residues. These act as docking sites for SH2 domain-mediated interactions with STAT proteins. Receptor-bound STATs are then phosphorylated on conserved Tyr residues within their C terminal domains prior to dissociation from the receptor and formation of homo- or heterodimers. These translocate to the nucleus, bind target gene promoters, and stimulate target gene transcription. (PMID: 30948191)

Publikationen

Species	Application	Title
human	WB	Front Immunol; Construction of disulfidptosis-based immune response prediction model with artificial intelligence and validation of the pivotal grouping oncogene c-MET in regulating T cell exhaustion; Authors - Pengping Li
mouse	WB	Int Immunopharmacol; Demethylzeylasteral alleviates inflammation and colitis via dual suppression of NF-κB and STAT3/5 by targeting IKKα/β and JAK2; Authors - Tian Wen
mouse	WB	CNS Neurosci Ther; Panaxadiol Attenuates Neuronal Oxidative Stress and Apoptosis in Cerebral Ischemia/Reperfusion Injury via Regulation of the JAK3/STAT3/HIF-1α Signaling Pathway; Authors - Jiabin Zhou