Phospho-JNK (Tyr185) Rekombinanter Antikörper
Phospho-JNK (Tyr185) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (3)
Anwendung
WB, IHC, IF/ICC, FC (Intra), ELISA
Konjugation
Unkonjugiert
CloneNo.
2G8
Kat-Nr. : 80024-1-RR
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293T-Zellen, mit Anisomycin behandelte NIH/3T3-Zellen, mit Calyculin A behandelte HEK-293T-Zellen, Mit UV behandelte HEK-293T-Zellen, Mit UV behandelte NIH/3T3-Zellen |
Erfolgreiche Detektion in IF/ICC | UV treated HEK-293 cells |
Erfolgreiche Detektion in FC (Intra) | Mit Calyculin A behandelte HEK-293-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 273 publications below |
IHC | See 5 publications below |
IF | See 8 publications below |
Produktinformation
80024-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Phospho-JNK (Tyr185) und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Rekombinant |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | mitogen-activated protein kinase 8 |
Beobachtetes Molekulargewicht | 37-46 kDa |
GenBank-Zugangsnummer | NM_139049 |
Gene symbol | JNK |
Gene ID (NCBI) | 5599 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Phospho-JNK (Tyr185) antibody 80024-1-RR | Protokoll herunterladen |
IF protocol for Phospho-JNK (Tyr185) antibody 80024-1-RR | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression | ||
Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response | ||
Clin Transl Med PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction | ||
J Nanobiotechnology Peptide-anchored neutrophil membrane-coated biomimetic nanodrug for targeted treatment of rheumatoid arthritis | ||
Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A | ||
J Neuroinflammation SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury |