Phospho-JNK (Tyr185) Rekombinanter Antikörper

Phospho-JNK (Tyr185) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(277)

CloneNo.

2G8

Kat-Nr. : 80024-1-RR

Synonyme

JNK (Tyr185), p JNK, p JNK (Tyr185), p JNK Tyr185, Phospho JNK



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293T-Zellen, mit Anisomycin behandelte NIH/3T3-Zellen, mit Calyculin A behandelte HEK-293T-Zellen, Mit UV behandelte HEK-293T-Zellen, Mit UV behandelte NIH/3T3-Zellen
Erfolgreiche Detektion in IF/ICCUV treated HEK-293 cells
Erfolgreiche Detektion in FC (Intra)Mit Calyculin A behandelte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

80024-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Phospho-JNK (Tyr185) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name mitogen-activated protein kinase 8
Beobachtetes Molekulargewicht37-46 kDa
GenBank-ZugangsnummerNM_139049
Gene symbol JNK
Gene ID (NCBI) 5599
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Phospho-JNK (Tyr185) antibody 80024-1-RRProtokoll herunterladen
IF protocol for Phospho-JNK (Tyr185) antibody 80024-1-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression

Authors - Xingran Li
mouseWB

Redox Biol

FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response

Authors - Zhe Wang
mouseWB

Clin Transl Med

PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction

Authors - Hao Zhang
mouseWB

J Nanobiotechnology

Peptide-anchored neutrophil membrane-coated biomimetic nanodrug for targeted treatment of rheumatoid arthritis

Authors - Ni Yang
humanWB

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
ratWB

J Neuroinflammation

SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury

Authors - Chenglong Wang