Phospho-JNK (Tyr185) Rekombinanter Antikörper

Phospho-JNK (Tyr185) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

131

CloneNo.

2G8

Kat-Nr. : 80024-1-RR

Synonyme

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, Phospho-JNK (Tyr185), PRKM8, SAPK1

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Galerie der Validierungsdaten

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  WB analysis using 80024-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80024-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80024-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, mit Anisomycin behandelte NIH/3T3-Zellen, mit Calyculin A behandelte HEK-293T-Zellen, Mit UV behandelte HEK-293T-Zellen, Mit UV behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 130 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

80024-1-RR bindet in WB, IHC, IF, ELISA Phospho-JNK (Tyr185) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 8
• Beobachtetes Molekulargewicht: 37-46 kDa
• GenBank-Zugangsnummer: NM_139049
• Gene symbol: MAPK8
• Gene ID (NCBI): 5599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
mouse	WB	J Nanobiotechnology; Peptide-anchored neutrophil membrane-coated biomimetic nanodrug for targeted treatment of rheumatoid arthritis; Authors - Ni Yang
human	WB	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
mouse	WB	J Hazard Mater; Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders.; Authors - Tianxin Zhao
human	WB	Cell Death Dis; USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1; Authors - Hongdian Zhang
mouse	WB	J Transl Med; Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways; Authors - Xin-Sen Chen