Phospho-JNK (Thr183/Tyr185) Monoklonaler Antikörper

Phospho-JNK (Thr183/Tyr185) Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2H8F12

Kat-Nr. : 60666-1-Ig

Synonyme

JNK (Thr183/Tyr185), Phospho-JNK, phospho-JNK(Thr183/Tyr185), phospho‐JNK/SAPK (Thr183/Tyr185), phospho-JNK1/2 (Thr183/Tyr185)

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  WB analysis using 60666-1-Ig

  Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as a loading control.

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  WB analysis using 60666-1-Ig

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

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  WB analysis using 60666-1-Ig

  Non-treated and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

• 

  WB analysis using 60666-1-Ig

  Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

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  IHC staining of Jurkat using 60666-1-Ig

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, NIH/3T3-Zellen, Mit UV behandelte NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Jurkat-Zellen; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

60666-1-Ig bindet in WB, IHC, ELISA Phospho-JNK (Thr183/Tyr185) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 8
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 50 kDa
• GenBank-Zugangsnummer: NM_138982
• Gene symbol: JNK
• Gene ID (NCBI): 5599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.

Publikationen

Species	Application	Title
mouse	WB	Mater Today Bio; Proanthocyanidin capsules remodel the ROS microenvironment via regulating MAPK signaling for accelerating diabetic wound healing; Authors - Shaoyin Wei
mouse	WB	Int Immunopharmacol; DPHB inhibits osteoclastogenesis by suppressing NF-κB and MAPK signaling and alleviates inflammatory bone destruction; Authors - Caixia Liu
human	WB	Cell Rep; High-glucose-associated YTHDC1 lactylation reduces the sensitivity of bladder cancer to enfortumab vedotin therapy; Authors - Zhuo Xing