Phospho-JNK (Thr183/Tyr185) Monoklonaler Antikörper

Phospho-JNK (Thr183/Tyr185) Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H8F12

Kat-Nr. : 60666-1-PBS

Synonyme

JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24

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  WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

  Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

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  WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

  Non-treated and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

  Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 60666-1-Ig (same clone as 60666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60666-1-PBS bindet in WB, IHC, Indirect ELISA Phospho-JNK (Thr183/Tyr185) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 8
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 50 kDa
• GenBank-Zugangsnummer: NM_138982
• Gene symbol: JNK
• Gene ID (NCBI): 5599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.