Phospho-JNK (Tyr185) Rekombinanter Antikörper

Phospho-JNK (Tyr185) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2G8

Kat-Nr. : 80024-1-PBS

Synonyme

JNK (Tyr185), p JNK , p JNK (Tyr185), p JNK Tyr185, Phospho JNK

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  WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

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  WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

  Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

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  WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

  Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 80024-1-RR (same clone as 80024-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed UV treated HEK-293 cells using Phospho-JNK (Tyr185) antibody (80024-1-RR, Clone: 2G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 80024-1-RR (same clone as 80024-1-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR, Clone:2G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

80024-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Phospho-JNK (Tyr185) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 8
• Beobachtetes Molekulargewicht: 37-46 kDa
• GenBank-Zugangsnummer: NM_139049
• Gene symbol: JNK
• Gene ID (NCBI): 5599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)

Publikationen

Species	Application	Title
human,rat	WB,IHC	Redox Rep; Selenium participates in the formation of kidney stones by alleviating endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells; Authors - Xiaozhe Su