Phospho-JUN (Ser63) Rekombinanter Antikörper

Phospho-JUN (Ser63) Rekombinant Antikörper für WB, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

240965B7

Kat-Nr. : 84147-1-RR

Synonyme

c-Jun (Ser63), P-cJun, p-c-Jun, p-c-Jun (Ser63), p-c-Jun(Ser63)

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  WB analysis using 84147-1-RR

  Non-treated NIH/3T3 cells, Anisomycin treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84147-1-RR (Phospho-JUN (Ser63) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  ChIP experiment of PC-12 using 84147-1-RR

  Chromatin was prepared from PC-12 cells starved overnight and treated with Human β-Nerve Growth Factor (hβ-NGF) (50 ng/ml, 2 hr). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-JUN (Ser63) (84147-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Anisomycin behandelte NIH/3T3-Zellen, Mit UV behandelte NIH/3T3-Zellen
• Erfolgreiche Detektion in ChIP-qPCR: hβ-NGF (50 ng/ml, 2 hr) PC-12 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84147-1-RR bindet in WB, ELISA, ChIP-qPCR Phospho-JUN (Ser63) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: jun oncogene
• Berechnetes Molekulargewicht: 331 aa, 36 kDa
• Beobachtetes Molekulargewicht: 40-48 kDa
• GenBank-Zugangsnummer: BC068522
• Gene symbol: JUN
• Gene ID (NCBI): 3725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)