Phospho-JUN (Ser73) Polyklonaler Antikörper

Phospho-JUN (Ser73) Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Kat-Nr. : 28891-1-AP

Synonyme

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Phospho-JUN (Ser73), Proto oncogene c Jun, Transcription factor AP 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMit UV behandelte NIH/3T3-Zellen
Erfolgreiche Detektion in IF/ICCNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28891-1-AP bindet in WB, IF/ICC, CoIP, ELISA Phospho-JUN (Ser73) und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name jun oncogene
Berechnetes Molekulargewicht 331 aa, 36 kDa
Beobachtetes Molekulargewicht38-45 kDa
GenBank-ZugangsnummerBC068522
Gene symbol JUN
Gene ID (NCBI) 3725
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Phospho-JUN (Ser73) antibody 28891-1-APProtokoll herunterladen
IF protocol for Phospho-JUN (Ser73) antibody 28891-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Small

Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy

Authors - Dan Li
WB,IF

J Cell Biol

JNK regulates ciliogenesis through the interflagellar transport complex and actin networks

Authors - Maria Chatzifrangkeskou
humanWB

J Cell Mol Med

SIAH2 suppresses c-JUN pathway by promoting the polyubiquitination and degradation of HBx in hepatocellular carcinoma

Authors - Qinghe Hu
mouseWB

Biochem Pharmacol

Activation of CXCL13/CXCR5 axis aggravates experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome.

Authors - Jiang Zhao
humanWB

Int J Biol Sci

RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.

Authors - Deyu Zhang
mouseWB

Front Pharmacol

Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis Partly by Inhibiting the Expression of LOX and LOXL-2.

Authors - Xiang Tong