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Phospho-JUN (Ser73) Polyklonaler Antikörper

Phospho-JUN (Ser73) Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28891-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-JUN (Ser73) Polyclonal antibody (28891-1-AP)

    WB analysis using 28891-1-AP (same clone as 28891-1-PBS)

    Non-treated NIH/3T3 and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28891-1-AP (Phospho-JUN (Ser73) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28891-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Phospho-JUN (Ser73) Polyclonal antibody (28891-1-AP)

    IF Staining of NIH/3T3 using 28891-1-AP (same clone as 28891-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed UV treated and non-treated NIH/3T3 cells using Phospho-JUN (Ser73) antibody (28891-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28891-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28891-1-PBS bindet in WB, IF/ICC, Indirect ELISA Phospho-JUN (Ser73) und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name jun oncogene
Berechnetes Molekulargewicht 331 aa, 36 kDa
Beobachtetes Molekulargewicht38-45 kDa
GenBank-ZugangsnummerBC068522
Gene symbol JUN
Gene ID (NCBI) 3725
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.