Phospho-JUN (Ser73) Rekombinanter Antikörper

Phospho-JUN (Ser73) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

4A18

Kat-Nr. : 80086-1-RR

Synonyme

JUN (Ser73), JUN (Thr91/93), p JUN , p JUN (Ser73), p JUN (Thr91/93)

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  WB analysis using 80086-1-RR

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80086-1-RR (Phospho-JUN (Ser73) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody (66009-1-Ig) as loading control.

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  IF Staining of HeLa using 80086-1-RR

  Immunofluorescent analysis of (4% PFA) fixed untreated A549 cells, UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-JUN (Ser73) antibody (80086-1-RR, Clone: 4A18) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004).

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  FC experiment of NIH/3T3 using 80086-1-RR

  1x10^6 NIH/3T3 cells untreated (dashed lines) or treated with UV which intracellularly stained with 0.13 ug Phospho-JUN (Ser73) Recombinant antibody (80086-1-RR, Clone:4A18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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  ChIP experiment of PC-12 using 80086-1-RR

  Chromatin was prepared from PC-12 cells starved overnight and treated with β-NGF (50 ng/ml) for 2 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-JUN (Ser73) (80086-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit UV behandelte NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells
• Erfolgreiche Detektion in FC (Intra): Mit UV behandelte NIH/3T3-Zellen
• Erfolgreiche Detektion in ChIP-qPCR: hβ-NGF (starved overnight) (50 ng/ml, 2 h) PC-12 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

80086-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR Phospho-JUN (Ser73) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: jun oncogene
• Berechnetes Molekulargewicht: 331 aa, 36 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: BC068522
• Gene symbol: JUN
• Gene ID (NCBI): 3725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.

Publikationen

Species	Application	Title
human,mouse	WB	Cell Host Microbe; Veillonella intestinal colonization promotes C. difficile infection in Crohn's disease; Authors - Ziyu Yang
human	WB,IHC	Int Immunopharmacol; Anti-osteosarcoma activity of Corynoline via Src/JNK signaling-mediated cell cycle arrest and mitochondrial apoptosis; Authors - Mufeng Li
mouse,human	WB,IHC	Chem Biol Interact; Pulsatilla saponin D inhibited the growth of osteosarcoma by regulating the JNK/ATF3 signaling pathway; Authors - Kaipeng Jin
chicken	WB	Vet Sci; Multi-Omics Unveils Inflammatory Regulation of Fermented Sini Decoction Dregs in Broilers Infected with Avian Pathogenic Escherichia coli; Authors - Shuanghao Mo
rat	WB	J Ethnopharmacol; Discovery and validation of anti-arthritic ingredients and mechanisms of Qingfu Juanbi Tang, a Chinese herbal formulation, on rheumatoid arthritis; Authors - Muzi Peng
mouse	WB	Heliyon; LINC02257 regulates colorectal cancer liver metastases through JNK pathway; Authors - Xiangan Wu