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Phospho-JUN (Thr91/93) Rekombinanter Antikörper

Phospho-JUN (Thr91/93) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241983H8

Kat-Nr. : CL647-81667-2

Synonyme

JUN (Thr91/93), p JUN (Thr91/93), Phospho JUN (Thr91/93), Phospho-JUN, Phospho-JUN Thr91/93

Filter:
  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite® Plus 647-conjugated Phospho-JUN (Thr91/9 (CL647-81667-2)

    FC experiment of NIH/3T3 using CL647-81667-2

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with UV which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-JUN (Thr91/93) Recombinant Antibody (CL647-81667-2, Clone:241983H8)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Mit UV behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-81667-2 bindet in FC (Intra) Phospho-JUN (Thr91/93) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name jun oncogene
Berechnetes Molekulargewicht 331 aa, 36 kDa
Beobachtetes Molekulargewicht48 kDa
GenBank-ZugangsnummerBC068522
Gene symbol JUN
Gene ID (NCBI) 3725
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)