Phospho-LCK (Tyr505) Rekombinanter Antikörper

Phospho-LCK (Tyr505) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242450G6

Kat-Nr. : 85679-1-PBS

Synonyme

LCK (Tyr505), p LCK, p LCK (Tyr505), Phospho LCK, Phospho LCK (Tyr505)

---

• 

  WB analysis using 85679-1-RR (same clone as 85679-1-PBS)

  Non-treated Jurkat cells and H2O2 treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85679-1-RR (Phospho-LCK (Tyr505) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85679-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

85679-1-PBS bindet in WB, Indirect ELISA Phospho-LCK (Tyr505) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lymphocyte-specific protein tyrosine kinase
• Berechnetes Molekulargewicht: 539 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC013200
• Gene symbol: LCK
• Gene ID (NCBI): 3932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lck is comprised of a SH3 domain, binding prolinerich regions, a SH2 domain, binding tyrosine-phosphorylated sequences, a kinase domain, a unique domain, and the negative regulatory tail. The kinase domain of Lck contains 2 important tyrosine residues. Tyr394 and Tyr505 represent the activating and inhibitory tyrosine residue, respectively. Therefore, Lck can exist in 4 distinct states of activity: an inactive state, a primed state, an active state, and a dually phosphorylated active state . Transitions between activity states are governed by phosphorylation at the Tyr394 and Tyr505 residues. When Tyr394 is dephosphorylated while Tyr505 is phosphorylated, Lck is in an inactive state. Lck becomes primed when Tyr505 is dephosphorylated and fully active after Tyr394 phosphorylation. In addition, there is a dually phosphorylated active form of Lck when both Tyr394 and Tyr505 residues are phosphorylated. (PMID: 32794043)