Phospho-MAP3K1 (Thr1400) Polyklonaler Antikörper

Phospho-MAP3K1 (Thr1400) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28844-1-AP

Synonyme

MAP3K1, MAPK/ERK kinase kinase 1, MAPKKK1, MEK kinase 1, MEKK, MEKK 1, MEKK1, Phospho-MAP3K1 (Thr1400)

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  WB analysis using 28844-1-AP

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28844-1-AP (Phospho-MAP3K1 (Thr1400) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

28844-1-AP bindet in WB, ELISA Phospho-MAP3K1 (Thr1400) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 1
• Berechnetes Molekulargewicht: 164 kDa
• Beobachtetes Molekulargewicht: 164 kDa
• GenBank-Zugangsnummer: NM_005921
• Gene symbol: MAP3K1
• Gene ID (NCBI): 4214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. MAP3K1 is activated by autophosphorylation on Thr-1400 and Thr-1412(Uniprot).

Publikationen

Species	Application	Title
rat	WB	Eur J Pharmacol; Resveratrol inhibits autophagy against myocardial ischemia-reperfusion injury through the DJ-1/MEKK1/JNK pathway; Authors - Song Liu
human	WB	Cell; Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket; Authors - Johannes Morstein
human	WB	Cell Death Dis; CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer; Authors - Cong Xiang