Phospho-TAK1 (Thr187) Polyklonaler Antikörper

Phospho-TAK1 (Thr187) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 28958-1-AP

Synonyme

MAP3K7, Phospho-MAP3K7/TAK1 (Thr187), TAK1, TGF beta activated kinase 1, TGF1a

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  WB analysis using 28958-1-AP

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28958-1-AP (Phospho-TAK1 (Thr187) antibody) at dilution of 1:5000 incubated 4℃ overnight. The membrane was stripped and reblotted with GAPDH antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Calyculin A behandelte HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

28958-1-AP bindet in WB, ELISA Phospho-TAK1 (Thr187) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 7
• Berechnetes Molekulargewicht: 579 aa, 64 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC017715
• Gene symbol: TAK1
• Gene ID (NCBI): 6885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily.It plays an important role in the cascades of cellular responses evoked by changes in the environment.It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825).It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Mol Cell Proteomics; Global Phosphoproteomics Unveils Kinase-regulated Networks in Systemic Lupus Erythematosus; Authors - Shuhui Meng
human	WB	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu
human	WB	Cell Mol Immunol; SMAD7 expression in CAR-T cells improves persistence and safety for solid tumors; Authors - Sixin Liang
human	WB	Cell Death Dis; CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer; Authors - Cong Xiang
mouse	WB	Acta Pharm Sin B; Free fatty acid receptor-4 regulates T-cell-mediated allogeneic reaction through activating an aryl hydrocarbon receptor pathway; Authors - Maxwell Duah