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Phospho-MEK1 (Thr286) Rekombinanter Antikörper

Phospho-MEK1 (Thr286) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241910G6

Kat-Nr. : 80629-2-PBS

Synonyme

MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2

Filter:
  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-MEK1 (Thr286) Recombinant antibody (80629-2-RR)

    WB analysis of NIH/3T3 using 80629-2-RR (same clone as 80629-2-PBS)

    Non-treated NIH/3T3 cells and nocodazole treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80629-2-RR (Phospho-MEK1 (Thr286) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80629-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

80629-2-PBS bindet in WB, Indirect ELISA Phospho-MEK1 (Thr286) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name mitogen-activated protein kinase kinase 1
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht40-45 kDa
GenBank-ZugangsnummerBC139729
Gene symbol MEK1
Gene ID (NCBI) 5604
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 enzymatic activity is regulated by site-specific phosphorylation that can be activated with phosphorylation of Ser217/Ser221 by Raf kinase or suppressed by phosphorylation of Thr286 and Thr292 by CDK1 and CDK5 or Thr292 and Thr386 by ERK1/2. (PMID: 31972311, PMID: 17928366, PMID: 22177953)