Phospho-MEK1 (Thr292) Monoklonaler Antikörper

Phospho-MEK1 (Thr292) Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D7A8

Kat-Nr. : 67873-1-PBS

Synonyme

MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2

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  WB analysis using 67873-1-Ig (same clone as 67873-1-PBS)

  Non-treated A431 and Nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 67873-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 67873-1-PBS in a different storage buffer formulation.

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  WB analysis using 67873-1-Ig (same clone as 67873-1-PBS)

  Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67873-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 67873-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67873-1-Ig (same clone as 67873-1-PBS)

  Non-treated cells and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 67873-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 67873-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67873-1-Ig (same clone as 67873-1-PBS)

  1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.13 ug Anti-Human Phospho-MEK1 (Thr292) (67873-1-Ig, Clone:2D7A8) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 67873-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67873-1-PBS bindet in WB, Indirect ELISA Phospho-MEK1 (Thr292) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC139729
• Gene symbol: MEK1
• Gene ID (NCBI): 5604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)