Phospho-MEK1 (Thr386) Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Phospho-MEK1 (Thr386) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

6K5

Kat-Nr. : 81304-1-RR

Synonyme

MEK1 (Thr386), p MEK1 , p MEK1 (Thr386), p MEK1 Thr386, Phospho MEK1

---

• 

  WB analysis of HeLa using 81304-1-RR

  Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 81304-1-RR (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis of HeLa using 81304-1-RR

  WB result of Phospho-MEK1 (Thr386) antibody (81304-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-MEK1 (Thr386) transfected HeLa cells.

• 

  IF Staining of HeLa using 81304-1-RR

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-MEK1 (Thr386) antibody (81304-1-RR, Clone: 6K5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 81304-1-RR

  1X10^6 HeLa cells (dashed untreated ines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-MEK1 (Thr386) Recombinant antibody (81304-1-RR, Clone:6K5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mit λ-Phosphatase behandelte HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: Mit λ-Phosphatase behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit λ-Phosphatase behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

81304-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA Phospho-MEK1 (Thr386) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC139729
• Gene symbol: MEK1
• Gene ID (NCBI): 5604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)

Publikationen

Species	Application	Title
human,mouse	WB	Cell Host Microbe; Veillonella intestinal colonization promotes C. difficile infection in Crohn's disease; Authors - Ziyu Yang
human	WB	Biomed Pharmacother; An anti-RAGE chimeric antibody alleviates CCl4-induced liver fibrosis via RAGE/NF-kB pathway in mice; Authors - Jing Liu