Phospho-MEK6 (Ser207) Rekombinanter Antikörper

Phospho-MEK6 (Ser207) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250293G8

Kat-Nr. : 80064-2-PBS

Synonyme

MKK6 (Ser207), Phospho-MEK6, Phospho-MEK6(Ser207), Phospho-MKK3 (Ser189), Phospho-MKK3 (Ser189)/MKK6 (Ser207)

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  WB analysis of HEK-293 using 80064-2-RR (same clone as 80064-2-PBS)

  Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80064-2-PBS in a different storage buffer formulation.

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  WB analysis using 80064-2-RR (same clone as 80064-2-PBS)

  Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80064-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80064-2-PBS bindet in WB, Indirect ELISA Phospho-MEK6 (Ser207) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase 6
• Berechnetes Molekulargewicht: 334 aa, 37 kDa
• Beobachtetes Molekulargewicht: 38 kDa, 40kDa
• GenBank-Zugangsnummer: BC012009
• Gene symbol: MEK6
• Gene ID (NCBI): 5608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). The antibody also recognizes the ser218 phosphorylation site of MEK3.