Phospho-MKK7 (Ser271/Thr275) Monoklonaler Antikörper

Phospho-MKK7 (Ser271/Thr275) Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B4B5

Kat-Nr. : 68050-1-PBS

Synonyme

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MKK7 (Ser271/Thr275), PRKMK7

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  WB analysis using 68050-1-Ig (same clone as 68050-1-PBS)

  Non-treated and Calyculin A treated, λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68050-1-Ig (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68050-1-PBS in a different storage buffer formulation.

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  WB analysis using 68050-1-Ig (same clone as 68050-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68050-1-Ig (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68050-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68050-1-PBS bindet in WB, Indirect ELISA Phospho-MKK7 (Ser271/Thr275) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase 7
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47-52 kDa
• GenBank-Zugangsnummer: BC038295
• Gene symbol: MKK7
• Gene ID (NCBI): 5609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)