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Phospho-MLKL (Ser358) Rekombinanter Antikörper

Phospho-MLKL (Ser358) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241469D2

Kat-Nr. : 82090-2-PBS

Synonyme

MLKL (Ser358), p MLKL (Ser358), Phospho-MLKL, Phospho-MLKL Ser358, phospho-Ser-358 MLKL

Filter:
  • Western Blot (WB) analysis of HT-29 cells using Phospho-MLKL (Ser358) Recombinant antibody (82090-2-RR)

    WB analysis of HT-29 using 82090-2-RR (same clone as 82090-2-PBS)

    Non-treated HT-29 cells, TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 82090-2-RR (Phospho-MLKL (Ser358) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82090-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

82090-2-PBS bindet in WB, Indirect ELISA Phospho-MLKL (Ser358) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name mixed lineage kinase domain-like
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht54 kDa
GenBank-ZugangsnummerXM_005255834
Gene symbol MLKL
Gene ID (NCBI) 197259
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily. One common method for inducing necroptosis is by treating cells grown in culture with a mixture composed of TNF-α, a Smac-mimetic, that degrades cellular inhibitors of apoptosis (cIAPs) and the pancaspase inhibitor Z-VAD-FMK. This mixture is abbreviated as T/S/Z. It drives necrosome complex formation and RIPK3-dependent phosphorylation of Thr-357 and Ser-358 located within the C-terminal kinase-like domain of human MLKL. (PMID: 28878015)