Phospho-Myosin Light Chain 2 (Thr18/Ser19) Polyklonaler Antikörper

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Polyklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 29504-1-AP

Synonyme

MYL12A, MYL12B, P MYL12A, P MYL12B, p MYL9

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  WB analysis using 29504-1-AP

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 29504-1-AP (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  FC experiment of HeLa using 29504-1-AP

  1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-Myosin Light Chain 2 (Thr18/Ser19) Polyclonal antibody (29504-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG control Polyclonal antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit λ-Phosphatase behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

29504-1-AP bindet in WB, IF, FC (Intra), ELISA Phospho-Myosin Light Chain 2 (Thr18/Ser19) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, light chain 9, regulatory
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 19-20 kDa
• GenBank-Zugangsnummer: BC002648
• Gene symbol: MYL9
• Gene ID (NCBI): 10398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.

Publikationen

Species	Application	Title
human	IF	Front Cell Dev Biol; Paracrine HB-EGF signaling reduce enhanced contractile and energetic state of activated decidual fibroblasts by rebalancing SRF-MRTF-TCF transcriptional axis; Authors - Junaid Afzal
human	WB	Int J Mol Sci; Non-Muscle MLCK Contributes to Endothelial Cell Hyper-Proliferation through the ERK Pathway as a Mechanism for Vascular Remodeling in Pulmonary Hypertension; Authors - Mariam Anis
human	WB	FASEB J; Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis; Authors - Xiang Gao
rat	WB	Am J Respir Cell Mol Biol; SOX17 Prevents Endothelial-Mesenchymal Transition of Pulmonary Arterial Endothelial Cells in Pulmonary Hypertension through Mediating TGF-β/Smad2/3 Signaling; Authors - Xiaozhou Zou
mouse,human	WB	Int J Biol Sci; Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis; Authors - Ding Yang