Phospho-Myosin Light Chain 2 (Thr18/Ser19) Rekombinanter Antikörper

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250098F12

Kat-Nr. : 85804-1-RR

Synonyme

MYL12B, MYL9, P MYL12A, P MYL12B, P Myosin Light Chain 2 (Thr18/Ser19)

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  WB analysis of HEK-293 using 85804-1-RR

  Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 85804-1-RR

  Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 85804-1-RR

  Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Calyculin A behandelte NIH/3T3-Zellen, Mit Calyculin A behandelte HEK-293-Zellen, mit Calyculin A behandelte HSC-T6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85804-1-RR bindet in WB, ELISA Phospho-Myosin Light Chain 2 (Thr18/Ser19) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, light chain 12A, regulatory, non-sarcomeric
• Berechnetes Molekulargewicht: 171 aa, 20 kDa
• Beobachtetes Molekulargewicht: 19-20 kDa
• GenBank-Zugangsnummer: BC016372
• Gene symbol: MYL12A
• Gene ID (NCBI): 10627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.