Phospho-NDRG1 (Thr346) Polyklonaler Antikörper

Phospho-NDRG1 (Thr346) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30886-1-AP

Synonyme

p NDRG1, p NDRG1 (Thr346), p NDRG1 Thr346, Phospho NDRG1, Phospho NDRG1 (Thr346)

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  WB analysis using 30886-1-AP

  Non-treated A431 cells, phosphatase inhibitor treated A431 cells and λ phosphatase treated A431 cells were subjected to SDS PAGE followed by western blot with 30886-1-AP (Phospho-NDRG1 (Thr346) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, λ phosphatase treated A431 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30886-1-AP bindet in WB, ELISA Phospho-NDRG1 (Thr346) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: N-myc downstream regulated 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 48 kDa
• GenBank-Zugangsnummer: BC006260
• Gene symbol: NDRG1
• Gene ID (NCBI): 10397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N-Myc Downstream Regulated 1 (NDRG1) is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. The phosphorylation of NDRG1 (on Thr346) showed the greatest statistically significant increase in clear cell renal cell carcinoma (ccRCC) compared with noncancerous renal tissue. Further study showed the phosphorylation of NDRG1 (Thr346) in ccRCC cells was maintained by RICTOR and mTOR in complex 2. (PMID: 37298315)