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Phospho-NDRG1 (Thr346) Rekombinanter Antikörper

Phospho-NDRG1 (Thr346) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250220C11

Kat-Nr. : 85141-5-PBS

Synonyme

NDRG1 (Thr346), p NDRG1, p NDRG1 (Thr346), Phospho NDRG1, Phospho NDRG1 (Thr346)

Filter:
  • Western Blot (WB) analysis of C2C12 cells using Phospho-NDRG1 (Thr346) Recombinant antibody (85141-5-RR)

    WB analysis of C2C12 using 85141-5-RR (same clone as 85141-5-PBS)

    Non-treated C2C12 cells and Insulin treated C2C12 cells were subjected to SDS PAGE followed by western blot with 85141-5-RR (Phospho-NDRG1 (Thr346) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85141-5-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85141-5-PBS bindet in WB, Indirect ELISA Phospho-NDRG1 (Thr346) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name N-myc downstream regulated 1
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht46 kDa, 48 kDa
GenBank-ZugangsnummerBC006260
Gene symbol NDRG1
Gene ID (NCBI) 10397
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N-Myc Downstream Regulated 1 (NDRG1) is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. The phosphorylation of NDRG1 (on Thr346) showed the greatest statistically significant increase in clear cell renal cell carcinoma (ccRCC) compared with noncancerous renal tissue. Further study showed the phosphorylation of NDRG1 (Thr346) in ccRCC cells was maintained by RICTOR and mTOR in complex 2. (PMID: 37298315)