Phospho-NF-κB p65 (Ser468) Rekombinanter Antikörper

Phospho-NF-κB p65 (Ser468) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

18

CloneNo.

6N1

Kat-Nr. : 82335-1-RR

Synonyme

NFKB3, p65, Phospho-NF-κB p65 (Ser468), RELA, Transcription factor p65

---

Galerie der Validierungsdaten

• 

  WB analysis using 82335-1-RR

  Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 82335-1-RR (Phospho-NF-κB p65 (Ser468) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin antibody (66031-1-Ig) and NF-κB p65 antibody (80979-1-RR) subsequently.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HeLa-Zellen, mit Calyculin A behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below

Produktinformation

82335-1-RR bindet in WB, ELISA Phospho-NF-κB p65 (Ser468) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC011603
• Gene symbol: RELA
• Gene ID (NCBI): 5970
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.

Publikationen

Species	Application	Title
	WB	Redox Biol; Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice; Authors - Boying Zhao
pig	WB	Int J Biol Macromol; Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway; Authors - Yuan-Feng Zou
human	WB	PLoS Pathog; Negative regulation of type I interferon signaling by integrin-linked kinase permits dengue virus replication; Authors - Yi-Sheng Kao
human	WB	Int Immunopharmacol; Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis; Authors - Wei Wu
human,rat	WB	Acta Biochim Biophys Sin (Shanghai); Mesenchymal stem cell exosomes inhibit nucleus pulposus cell apoptosis via the miR-125b-5p/TRAF6/NF-κB pathway axis; Authors - Yang Duan
rat	WB	Brain Behav; L-F001, a multifunctional fasudil-lipoic acid dimer, antagonizes hypoxic-ischemic brain damage by inhibiting the TLR4/MyD88 signaling pathway; Authors - Ruiyu Zhou