Phospho-NF-κB p65 (Ser536) Rekombinanter Antikörper

Phospho-NF-κB p65 (Ser536) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

240777D9

Rezensionen (1)

Kat-Nr. : 80379-2-RR

Synonyme

NF-κB p65 (Ser536), NF-κB p65 Ser-536, Phospho NF-κB p65 (Ser536), phospho-NF-κB p65, Phospho-NF-κB p65 Ser536

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• 

  WB analysis of NIH/3T3 using 80379-2-RR

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80379-2-RR (Phospho-NF-κB p65 (Ser536) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

• 

  WB analysis using 80379-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80379-2-RR (Phospho-NF-κB p65 (Ser536) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of PC-3 using 80379-2-RR

  1x10^6 untreated or Calyculin A treated PC-3 cells were intracellularly stained with 0.25 ug Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR, Clone:240777D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue), 1x10^6 untreated PC-3 cells were intracellularly stained with 0.25 ug Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR, Clone:240777D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(black). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Calyculin A behandelte NIH/3T3-Zellen, Mit Calyculin A behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below

Produktinformation

80379-2-RR bindet in WB, FC (Intra), ELISA Phospho-NF-κB p65 (Ser536) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC011603
• Gene symbol: NF-κB p65
• Gene ID (NCBI): 5970
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.

Publikationen

Species	Application	Title
mouse	WB	Int J Nanomedicine; Unexpected Inhibitory Role of Silica Nanoparticles on Lung Cancer Development by Promoting M1 Polarization of Macrophages; Authors - Meng Xiang
human	WB	Front Immunol; SQLE-mediated squalene metabolism promotes tumor immune evasion in pancreatic cancer; Authors - Junchen Pan
mouse	WB	J Ethnopharmacol; Proteomic analysis reveals that Acalypha australis L. mitigates chronic colitis by modulating the FABP4/PPARγ/NF-κB signaling pathway; Authors - Xiaoyu Quan
mouse,rat	WB	Cell Death Discov; 17β-estradiol alleviated ferroptotic neuroinflammation by suppressing ATF4 in mouse model of Parkinson's disease; Authors - Guoming Wang
mouse	WB	Adv Mater; Suppression of Sepsis Cytokine Storm by Escherichia Coli Cell Wall-Derived Carbon Dots; Authors - Yinan Li
mouse	WB	Clin Exp Pharmacol Physiol; Inhibition of DNA Methyltransferase DNMT1 Reverses Th2 Response Polarisation and Alleviates Allergic Rhinitis; Authors - Peng Zou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ana (Verified Customer) (07-10-2025)

No issues at all. Diluted it in 3% BSA in Tween 1%/PBS and detection was clear

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 4T1 cells

Phospho-NF-κB p65 (Ser536) Antibody Western Blot validation (1:1000 dilution) in 4T1 cells (Cat no:80379-2-RR)