Phospho-eNOS (Thr495) Polyklonaler Antikörper

Phospho-eNOS (Thr495) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 28939-1-AP

Synonyme

eNOS (Thr494), eNOS (Thr495), p eNOS , p eNOS (Thr495), p eNOS Thr495

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  WB analysis using 28939-1-AP

  Non-treated, VEGF (HZ-1038), EGF and Cobalt Chloride treated HUVEC cells were subjected to SDS PAGE followed by western blot with 28939-1-AP (Phospho-NOS3 (Thr495) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit VEGF behandelte HUVEC-Zellen, mit Cobaltchlorid behandelte HUVEC-Zellen, mit EGF behandelte HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

28939-1-AP bindet in WB, ELISA Phospho-eNOS (Thr495) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nitric oxide synthase 3 (endothelial cell)
• Berechnetes Molekulargewicht: 133 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC063294
• Gene symbol: eNOS
• Gene ID (NCBI): 4846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine. The sequence homology of eNOS protein in human and mouse is above 90%, and phosphorylation of threonine at 495 site in human corresponds to 494 site in mouse.

Publikationen

Species	Application	Title
human	WB	Hypertension; Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia; Authors - Lunbo Tan
mouse,human	WB	Commun Biol; Targeting GPR39 in structure-based drug discovery reduces Ang II-induced hypertension; Authors - Dongxu Hua
rat	WB	J Ethnopharmacol; Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway; Authors - Chuankui Fu
rat	WB	Drug Des Devel Ther; Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157.; Authors - Hailu Wu
human	WB	J Oncol; Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway.; Authors - Sha Wang
human	WB	Cytotherapy; Porous gelatin microspheres implanted with adipose mesenchymal stromal cells promote angiogenesis via protein kinase B/endothelial nitric oxide synthase signaling pathway in bladder reconstruction; Authors - Jun Zhao