Phospho-TrkA (Tyr496)/TrkB (Tyr516) Rekombinanter Antikörper

Phospho-TrkA (Tyr496)/TrkB (Tyr516) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241917B1

Kat-Nr. : 84976-1-RR

Synonyme

Phospho-NTRK1, NTRK1 (Tyr496), Phospho-NTRK1 (Tyr496), Phospho-NTRK1-Tyr496, Phospho-TrkA

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  WB analysis using 84976-1-RR

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 84976-1-RR (Phospho-NTRK1 (Tyr496) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: pervanadate treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84976-1-RR bindet in WB, ELISA Phospho-TrkA (Tyr496)/TrkB (Tyr516) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: neurotrophic tyrosine kinase, receptor, type 1
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC062580
• Gene symbol: NTRK1
• Gene ID (NCBI): 4914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The tyrosine kinase receptor TrkA (or neurotrophin receptor kinase 1, NTRK1) has been predominately studied for its role during the development of the nervous system. In developing neurons, TrkA activation upon binding of its ligands nerve growth factor (NGF) or neurotrophin-3 (NT-3) results in the stimulation of various tyrosine kinase-induced signaling pathways leading to neuronal outgrowth. (PMID: 32957504) This antibody recognizes the phosphorylation of TrkA at site 496, which is the phosphorylation of the TrkA-I(isoform 2) sequence at site 490.