Phospho-P53 (Ser15) Polyklonaler Antikörper

Phospho-P53 (Ser15) Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(35)

Kat-Nr. : 28961-1-AP

Synonyme

TP53, P53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53

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  WB analysis using 28961-1-AP

  Non-treated HT-29 and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53(Ser15) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of HT-29 using 28961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed non-treated HT-29 and etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HT-29 using 28961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HT-29 using 28961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Etoposid behandelte HT-29-Zellen
• Erfolgreiche Detektion in IF/ICC: mit Etoposid behandelte HT-29-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 32 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

28961-1-AP bindet in WB, IHC, IF/ICC, ELISA Phospho-P53 (Ser15) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p53
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003596
• Gene symbol: P53
• Gene ID (NCBI): 7157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.

Publikationen

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation; Authors - Huan Li
mouse	WB	Aging Cell; Excessive processing and acetylation of OPA1 aggravate age-related hearing loss via the dysregulation of mitochondrial dynamics; Authors - Andi Zhang
mouse	IF	Acta Pharmacol Sin; Exosomes are involved in total body irradiation-induced intestinal injury in mice.; Authors - Hang Li
human	WB	Cancer Cell Int; Marine natural product Methyl mycophenolate inhibits gastric cancer growth through regulating p53 and the downstream pathways; Authors - Xiaofang Liu
human	WB	J Nutr Biochem; Lycopene suppresses gastric cancer cell growth without affecting normal gastric epithelial cells; Authors - Ying Zhou
human	WB	Clin Epigenetics; Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line; Authors - Tina Aumer