Phospho-P53 (Ser15) Monoklonaler Antikörper

Phospho-P53 (Ser15) Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(18)

CloneNo.

1H6G1

Kat-Nr. : 67826-1-Ig

Synonyme

p P53, p P53 (Ser15), p P53 Ser15, P53 (Ser15), Phospho P53

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  WB analysis using 67826-1-Ig

  Non-treated and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

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  WB analysis using 67826-1-Ig

  Non-treated and Etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

• 

  WB analysis using 67826-1-Ig

  Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

• 

  WB analysis using 67826-1-Ig

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

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  IF Staining of HT-29 using 67826-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (67826-1-Ig, Clone: 1H6G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HT-29 using 67826-1-Ig

  1X10^6 HT-29 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (67826-1-Ig, Clone:1H6G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Calyculin A behandelte HT-29-Zellen, Mit Calyculin A behandelte HEK-293-Zellen, HEK-293-Zellen, HEK-293T-Zellen, HT-29-Zellen, Mit UV behandelte HEK-293T-Zellen
• Erfolgreiche Detektion in IF/ICC: mit Etoposid behandelte HT-29-Zellen
• Erfolgreiche Detektion in FC (Intra): UV treated HT-29 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

67826-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Phospho-P53 (Ser15) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p53
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003596
• Gene symbol: P53
• Gene ID (NCBI): 7157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Publikationen

Species	Application	Title
mouse	WB,IHC	Phytomedicine; MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice; Authors - Jia Shi
human	WB	J Virol; Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription; Authors - Xiazhen Xu
pig,mouse	WB	Food Chem Toxicol; Enhanced glutathione production protects against zearalenone-induced oxidative stress and ferroptosis in female reproductive system; Authors - Wei Fu
human	WB	Front Pharmacol; A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression.; Authors - Ting Wang
human	WB	Toxicol Lett; The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs]; Authors - Xiaoran Guan
rat	WB	World Neurosurg; N-acetylserotonin protects rat nucleus pulposus cells against oxidative stress injury by activating the PI3K/AKT signaling pathway; Authors - Wang Yidian